Archive | February, 2013

Get Payment From Regnow Affiliate?

My balance already over $500 on regnow affiliate account. How to get payment from ? Please tell me if anybody know about this.

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Really Need Help! Biotechnology Questions? Ap Bio?

1. You are a molecular biologist working at a biotech company. Your CEO has identified an untapped niche market — Canadian bachelor farmers — and thinks the company can make millions
developing new crops to sell to them. Your assignment is to develop, for the pickle industry, frost resistant cucumbers that glow fluorescent green so they are easy to find in the dark (short days in
northern Canada, you know). You have identified the Northern cod as a fish species that produces the appropriate anti-freeze protein (AFP). You are also able to buy a plasmid from BioRad
Laboratories containing the gene from a jellyfish species that produces a glowing fluorescent protein (GFP). You obtain Arctic cod and raise them in an aquarium in the lab. Your first step is to make a
genomic DNA library from the cod. Describe this process. Be specific!
2. Now you want to find the AFP gene in this cod genomic DNA library. Although the genome of the Arctic cod has not been sequenced, from careful study of GenBank, the online database of
DNA sequences, you are able to create a probe for the AFP gene from other arctic fish species. Explain what a probe is, and describe how you will use this to isolate the AFP gene from the
DNA library. Be specific!
3. You have now isolated the bacterial colony which contains the plasmid with a DNA fragment that includes the cod AFP gene. As a research control, you need to double check the size of
that cod DNA fragment. Describe a procedure for cutting out the cod DNA fragment from the plasmid and measuring its size.
4. Once you isolate the bacterial colony containing the plasmid with the cod AFP gene, you need to combine this gene with the jellyfish GFP gene on a single plasmid. As is standard practice in
the biotech industry, the GFP plasmid has a multiple cloning site and a selectable marker. Describe this cloning process. Be specific!
5. Research is going well! You are now working on overcoming the obstacle of getting your recombinant plasmid into the pickling cucumbers and getting high expression of both AFP and
GFP. Unfortunately, one night someone breaks into your lab and steals your most promising prototype cucumber seedlings. Your company’s security force calls in the CSI New York team
and they find a single hair follicle at the crime scene. How will they use this to find the thief? Be specific!

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Can I Apply To John Abbott College And Vanier College (montreal)?

My friend told me that I can’t apply to both John abbott and Vanier college because they are both affiliated with the SRAM or whatever… She said I will have to apply to one of them IN PERSON if i want to apply to both, and that i cannot apply to both of them online. IS this true???
thanks!!

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I Need Help Starting My Youtube Channel?

Please go and look at my videos, then subscribe if you like. Funnier ones will be coming soon. The channel name is CarzyGamerRush

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Going To Hong Kong, And I Want To Get My Nails Done; Where Should I Go? Where Else Should I Visit?

Hi there!
I’m going to Hong Kong late March and during my trip I want to get my nails done. Acrylic or gel, either or.
My hotel is the Harbour Plaza North Point Hotel; I think it’s very central, am I correct? My father will be attending the APLF, so we had to choose the hotel accordingly.
I’ve found some places online, but I’d like recommendations too. It would be great if you could give me an estimated price range.
Also, where else should I go for some cheap shopping? Alas, I’m not an itsy bitsy girl, so I doubt I’ll find any clothing my size, but accessories and little nik-naks
I’ll be traveling alone, so what else would you suggest? I’m in contact with one of the best travel agencies there [I also work in a travel agency in Istanbul 🙂 ] but I’d like the opinions of past travelers.
Oh, I’m a huge foodie. FOoOooOOood ♥♥♥ please ‘gimme ideas!
Ah and I wan’t to buy a rice cooker…how much do you reckon they’d go for? Where should I go?
Thnx!

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Best Gaming Affiliate Programs?

I’ve built a gaming website dedicated to Free-to-Play games. I’m looking for good gaming affiliate programs to advertise on the site. Affiliate programs for Free-to-Play games would be great but regular games, websites or accesories will do fine.
Any suggestions?

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